Fig. 7.

Xrn1 functions in mRNA transcription, decay, and translation are linked. a Transcription rates and b half-lives of the studied mRNA groups. Statistical significance was calculated using a Wilcoxon-test (*represents p-value < 0.001) The results represent an average of n = 3 biological replicates. For boxplots, box boundaries represent the 1st and 3rd quartile of the distribution, while the center line represents the 2nd quartile (median). Whiskers indicate either the most extreme values or extend to 1.5 times the interquartile range starting from the respective box boundary. c Xrn1 D208A effect on BMV RNA2 translation. d When introduced directly into the cytosol by electroporation, RNA2 does not depend on Xrn1 for translation. RNA2-Rluc mRNA and a control mRNA expressing Fluc were directly electroporated into the cytosol. 2a-Rluc protein values were normalized by those of Fluc and represented in comparison to the WT. Quality controls for RNA integrity and capping were performed (Supplementary Fig. 12). The results represent an average of n = 3 biological replicates ± SEM. Open circles indicate the individual dot plots. Source data are provided as a Source Data file