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. 2019 Feb 5;116(6):1025–1036. doi: 10.1016/j.bpj.2019.01.035

Figure 3.

Figure 3

Dissociation kinetics of Syt-1 C2 domains on (A) 1:1 DOPC/DOPS liposomes and (B) PM(−)PIP2 liposomes. Dansyl-PE fluorescence was monitored as solutions containing 5 μM (single domains) or 1 μM (tandem domain) protein, 200 μM CaCl2, and liposomes (200 μM accessible lipid) were rapidly mixed with an equal volume of 2 mM EDTA (all concentrations listed are before mixing). Kinetic data were fitted to Eqs. 2 or 3, with rate constants shown in Table 3. Inset shows the full timescale of measurement of Syt-1 C2AB. Data shown are representative of ≥3 independent measurements.