Figure 5.

Membrane insertion of Syt-7 C2A and C2B domain loops in the absence of PIP₂. Fluorescence emission spectra are shown of Syt-7 single domains (A, C, E, and G) or the C2AB tandem (B, D, F, and H) labeled with AEDANS on CBL1 (A, B, E, and F) or CBL3 (C, D, G, and H) (see Fig. S9 for schematic of labeling sites and Materials and Methods for residue numbers). Data are of protein in solution alone (black) and after addition of PM(−)PIP₂ liposomes (gray). All spectra are normalized to the maximal intensity in the absence of lipid.