Table 1.
Lipid Compositions Used in This Study
| Target Membrane Lipid Compositions (mol %) |
||||||||
|---|---|---|---|---|---|---|---|---|
| Name | PE | PCa | PSa | PI | PIP2 | Sphingomyelin | Cholesterol | Dansyl-PEb |
| PM | 27.9 | 10.7 | 21.3 | 3.6c | 2.0c | 4.5 | 25 | 5.0 |
| PM(−)PIP2 | 27.9 | 10.7 | 21.3 | 5.6 | – | 4.5 | 25 | 5.0 |
| 1:1 DOPC/DOPS | – | 47.5 | 47.5 | – | – | – | – | 5.0 |
a
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine were used for the physiological membranes, whereas DOPC and DOPS were used for the simplified lipid composition for consistency with prior experiments (44).
b
For AEDANS fluorescence measurements, dansyl-PE was omitted and replaced with PC.
c
For Ca2+ titrations, PM liposomes contained 4.6% PI and 1.0% PIP2.