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. 2019 Feb 5;116(6):1025–1036. doi: 10.1016/j.bpj.2019.01.035

Table 3.

Dissociation Kinetics of Syt-1 and Syt-7 C2 Domains

Domain koff, 1:1 DOPC/DOPS (s−1) koff, PM(−)PIP2 (s−1)
Syt-1 C2A 710 ± 30 1020 ± 90
Syt-1 C2B 140 ± 10 170 ± 10
Syt-1 C2AB 21 ± 2 (72% amp) 2.6 ± 0.1 (28% amp) 48 ± 5
Syt-7 C2A 6.39 ± 0.04 7.4 ± 0.3
Syt-7 C2B 80 ± 9 (88% amp) 13 ± 2 (12% amp) 100 ± 20
Syt-7 C2AB 4.6 ± 0.3 (74% amp) 0.16 ± 0.04 (26% amp) 5.9 ± 0.6 (77% amp) 0.64 ± 0.09 (23% amp)

Uncertainties represent SD from ≥ 3 separate experiments. Double-exponential rate constants are reported with their respective percent amplitudes from the best-fit curve.