Table 2.

List of mutants with phenotypic predictions that are inconsistent with observed phenotypes

Mutant	Arrest point	DNA	Nuclei	Comment	Synthetic lethality
cln3	lic, sat, 0	1	1	G1-arrest	cln3bck2
cln3mbp1	lic, sat, 0	1	1	G1-arrest	cln3bck2
cln3swi6	lic, sat, 0	1	1	G1-arrest	cln3bck2
cln3swi4	lic, sat, 0	1	1	G1-arrest	cln3bck2
swi4	lic, sat, large	1	1	G1-arrest
swi6	lic, sat, large	1	1	G1-arrest
swi64A	lic, sat, large	1	1	G1-arrest
mbp1	lic, sat, large	1	1	G1-arrest
clb5	lic, sat, large	1	1	G1-arrest	clb3clb4clb5
cdc20pds1clb5	lic, sat, large	1	1	G1-arrest	clb3clb4clb5
cdc20pds1clb5cdh1SIC1	lic, sat, large	1	1	G1-arrest	clb3clb4clb5
spc1102ACDK	lic, sat, large	1	1	G1-arrest
Sld2A	lic, sat, large	1	1	G1-arrest
Sld3T600A	lic, sat, large	1	1	G1-arrest
Sld3S622A	lic, sat, large	1	1	G1-arrest
Sic17A	lic, sat, large	1	1	G1-arrest
cdh1	0, sat, 0	1	1	G1-arrest
cln1cln2cdh1	0, sat, 0	1	1	G1-arrest
spc1104A	rep’d, dup, large	1	1	G2/M-arrest
pds1	0, 0, large	2	2	T arrest
net1cdc15cdh1	0, sat, large	2	2	T arrest
cdc15net1	?,?, large	*	*	Multinucleate: DNA replication and ND, no cell division
tem1net1	?,?, large	*	*	Multinucleate: DNA replication and ND, no cell division

The model predicted 23 viable mutants to be lethal. Of these, seven mutants can be explained by implicit synthetic lethality: while cln3 and clb5 are viable, the model lacks implementation of mechanistic functions of Bck2 and Clb3/Clb4, which are essential in the absence of Cln3 and Clb5, respectively. While other mutants have strong phenotypes in, e.g. cell size or growth rate, such as swi4, swi6 and pds1, others—like cdh1—have no strong phenotypes as single mutations, suggesting that redundancy mechanisms are missing in our current knowledge