Table 1.

Detection Results

Background	Jaccard	FP Rate	FN Rate
Dark counts	0.903	0.014	0.086
Dark counts + NPs	0.905	0.001	0.089
Dark counts + NPs + laser spot	0.878	0.016	0.100

The results show the Jaccard index, false-positive (FP) error rate, and false-negative (FN) error rate for single molecules in the different simulated movies of Fig. 2.