. 2019 Feb 16;116(6):975–982. doi: 10.1016/j.bpj.2019.02.006

Table 2.

Error Distribution Characteristics

Background	x Position Error (nm)^a	y Position Error (nm)^a	Intensity Error (%)^b
Dark counts	μ = 0.165	μ = 0.114	μ = 0.821
Dark counts	σ = 13.7	σ = 13.6	σ = 20.6
Dark counts + NPs	μ = –0.112	μ = –0.029	μ = 1.12
Dark counts + NPs	σ = 13.7	σ = 13.9	σ = 20.5
Dark counts + NPs + laser spot	μ = –0.050	μ = –0.185	μ = 1.64
Dark counts + NPs + laser spot	σ = 14.3	σ = 14.4	σ = 21.2

The characteristics mean, μ, and SD, σ, for the simulated movies are shown.

x and y position error is the difference between the measured and true positions of the molecule.

The intensity percent error is {100% × (measured − true)/true} for the summed pixel intensities in the local region around the molecule.