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. 2019 Mar 9;13:402–415. doi: 10.1016/j.isci.2019.03.005

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Y14 Associates with Chromatin and Accumulates on Chromatin after DNA Damage

(A) Immunoblotting was performed using the whole-cell extract (WCE) and RNase A-treated (+) and non-treated (−) chromatin fractions (CSK) of HeLa cells.

(B) HeLa cells were mock-irradiated (−) or irradiated with 10 Gy followed by recovery for 0, 1, or 2 h. Immunoblotting shows the indicated proteins in the WCE and RNase-treated (CSK + R) and non-treated (CSK) chromatin fractions.

(C) HeLa cells were mock-treated or treated with KU55933 followed by IR irradiation and subsequent recovery. The WCE and RNase-treated chromatin fraction (CSK + R) were subjected to immunoblotting. Bar graph shows the relative level of chromatin-bound Y14 without (lanes 9–12) or after KU55933 treatment (lanes 13–16); lane 9 was set as 1.