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. 2019 Jan 29;16(3):384–393. doi: 10.7150/ijms.30084

Figure 5.

Figure 5

PM2.5 increased the expression of miR-146a-3p and SIRT1 was a potential target of miR-146a-3p. (A) RAW264.7 cells were treated with 0, 25, 50, 100 μg/ml PM2.5 for 24h. The mRNA expression of miR-146a-3p was detected by qRT-PCR. (B-C) Relative expression of miR-146a-3p normalized against the U6 endogenous control and SIRT1 mRNA normalized against the β-actin endogenous control in untreated RAW264.7 transfected with miR-146a-3p mimic, inhibitor or scrambled controls. (D) Wild-type and mutant binding sites of miR-146a-3p in the 3′-UTR of SIRT1. (E) Luciferase analysis. The results showed that miR-146a-3p mimics decreased the fluorescence intensity in cells transfected with SIRT1-3′-UTR-wt but did not change the fluorescence intensity in cells transfected with SIRT1-3′-UTR-mut. (F) Western blotting analysis of SIRT1 in untreated RAW264.7 transfected with miR-146a-3p mimic, inhibitor or scrambled controls. *p<0.05, **p<0.01, ***p<0.001 versus control group or between indicated groups.