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. 2019 Feb 19;15(4):812–825. doi: 10.7150/ijbs.31335

Figure 6.

Figure 6

MMP28 promoted EMT, migration and invasion of HCC cells via activating Notch3 signals. (a) Levels of NICD3, NICD4, Akt, p-Akt(S), p-Akt(T), PKG-I, VASP, p(S239)-VASP and β-catenin in MMP28-overexpressing 7402 cells were determined by western blot. (b) The mRNA levels of Notch3, Notch4, Akt, PKG1, VASP and β-catenin in MMP28-overexpressing 7402 cells was determined by real-time PCR. (c) The expression of MMP28 and NICD3 in MMP28-knockdowned Huh7 cells were determined by western blot. (d) Expression correlation between MMP28 and Notch3 from TCGA dataset. (e) Correlation of MMP28 mRNA levels with Notch3 in HCC tissues was determined by real-time PCR. (f-g) Correlation of MMP28 mRNA levels with Notch3 in IHC cohort was determined by IHC analysis. Representative images of MMP28 and Notch3 in HCC tissues were shown. *P < 0.05; **P < 0.01; ***P < 0.001. Data are represented as Means ± SD.