Figure 8.

The Ca²⁺ response in the stratified eggs at fertilization. (A) Pseudo-colored images showing the CF and the beginning of the sperm-initiated Ca²⁺ wave in intact and centrifuged eggs of P. lividus. Note the secondary calcium increase during the CF (at 5.3 sec) in the centrifugal pole of the centrifuged egg (arrow). Scale bar 20 µm. (B) Comparison of various aspects of the Ca²⁺ signaling in the intact and stratified eggs. See the text in Results for detail. (C) Calcium curves at fertilization in intact (green) and stratified eggs (brown) from one batch of experiments demonstrating the suppression of the Ca²⁺ wave in stratified eggs (brown).