Table 1.
Treatment | Microvilli shape | Average microvilli count per treatment * | Comparison with control eggs | ||
---|---|---|---|---|---|
Normal (%) | Abnormal (%) | Shape change | counts | ||
FSW (control) | 99.06 | 0.94 | 179.9 (n=4) | n/a | n/a |
Urethane 400 mM | 88.96 | 11.04 | 118.7 (n=4) | P < 0.001 | P < 0.001 |
Procaine 10 mM | 82.39 | 17.61 | 112.3 (n=4) | P < 0.001 | P < 0.001 |
NH4Cl 40 mM | 88.61 | 11.39 | 132.5 (n=4) | P < 0.001 | P < 0.01 |
Stratified | 83.16 | 16.84 | 84.6 (n=4) | P < 0.001 | P < 0.001 |
DMSO (control) | 98.51 | 1.49 | 230.1 (n=4) | n/a | n/a |
GPN 200 µM | 85.88 | 14.12 | 112.1 (n=4) | P < 0.001 | P < 0.001 |
FSW (control) | 99.64 | 0.36 | 324.83 (n=2) | n/a | n/a |
Diltiazem 50 µM | 94.1 | 5.82 | 265.91 (n=4) | P < 0.01 | P < 0.05 |
Verapamil 50 µM | 91.95 | 8.05 | 273.5 (n=4) | P < 0.01 | P < 0.05 |
* Microvilli counted from three separate regions (26.1 μm2) randomly selected from 2 to 4 different eggs for each experiment. n/a, nonapplicable.