Table 2.

Intracellular Ca²⁺ increase in fertilized sea urchin eggs pretreated with urethane, procaine and NH₄Cl.

	Fertilization				Ca2+ decline level (RFU)	VL Elevation %
	Cortical flash	Latent period (sec)	Global Ca2+ (RFU)	Planar velocity (µm/sec)	3 min post-fertilization	Full	Partial	None	n
Control (FSW)	0.068 ± 0.023	8.4955 ± 4.755	0.445 ± 0.097	5.0204 ± 0.6601	0.2808 ± 0.0623	60.8	30.5	8.7	23
Urethane 400 mM	0.051 ± 0.017	7.0422 ± 5.2904	0.4204 ± 0.075	5.8657 ± 1.2344	0.2141 ± 0.0739	86.3	9.1	4.6	22
T-test	< 0.05	0.3374	0.341	< 0.01	< 0.01
Control (FSW)	0.087 ± 0.013	10.33 ± 4.736	0.412 ± 0.066	5.0447 ± 2.0975	0.2769 ± 0.0675	72.7	27.3	0	11
Procaine 10 mM	0.0347 ± 0.017	11.125 ± 5.15	0.472 ± 0.081	3.2696 ± 0.3560	0.1231 ± 0.1033	0	0	100	9
T-test	< 0.001	0.7236	< 0.05	< 0.05	P< 0.001
Control (FSW)	0.066 ± 0.016	8.934 ± 2.396	0.361 ± 0.049	3.4338 ± 0.5819	0.227 ± 0.042	72.7	18.2	9.1	11
NH4Cl 40 mM	0.042 ± 0.028	8.415 ± 4.095	0.348 ± 0.088	3.4263 ± 0.9704	0.0816 ± 0.0257	0	44.4	55.6	9
T-test	< 0.05	0.7279	0.6764	0.9832	< 0.001