Fig 4.

Effect of farrerol on H₂O₂-induced cell viability and oxidative stress. (A) HepG2 cells were treated with increasing concentrations of farrerol (5, 10 and 20 μM) for 1 h, followed by treatment with H₂O₂ (300 μM) for 24 h, and then cell viability was evaluated by MTT assay. (B) HepG2 cells were cultured with farrerol (5, 10 and 20 μM) for 18 h, followed by exposure to H₂O₂ (300 μM) for 5 min, and then the cells were harvested and were stained with 50 mM of DCFH-DA for 30 min to induce ROS generation. All of the data shown represent the average from three independent experiments. *p < 0.05 and **p < 0.01 versus the H₂O₂ group.