Fig 7.

The activation of autophagy by farrerol is necessary for cytoprotection. (A-B) HepG2 cells were incubated with farrerol for 18 h, and then the expression of Atg5, Atg7 and LC3 were detected by immunoblotting by using specific antibodies. (C-D) Cells were treated with 20 μM CQ for 1 h, followed by treatment with farrerol for 18 h, and then cell lysates were immunoblotted to assess Atg5, Atg7 and LC3 expression. (E) Cells were preincubated with CQ (20 μM) for 1 h, incubated with farrerol for another 1 h, and then incubated with H₂O₂ (300 μM) for 24 h. Subsequently, cell viability was assessed by MTT assay. All of the data shown represent the average from three independent experiments. *p < 0.05 and **p < 0.01 versus the control or H₂O₂ group.