Fig 8.

The suppressive effects of farrerol on APAP-induced liver injury depended on Nrf2 and autophagy. (A) The survival rates of the WT and Nrf2 ^-/- mice (n = 10/group) were observed within 48 h after APAP exposure. (B-C) Serum samples were collected from the mice after exposure to APAP (400 mg/kg) for 6 h in order to measure the ALT and AST levels. (D) Livers from each experimental group were processed for histological evaluation at 6 h after the APAP (400 mg/kg) challenge. (E) Livers from each experimental group were immunoblotted to assess Nrf2, JNK, Atg5, Atg7 and LC3 expression. All of the data shown represent the average from three independent experiments. *p < 0.05 and **p < 0.01 versus the APAP group.