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. 2019 Mar 15;10:265. doi: 10.3389/fpls.2019.00265

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Copyright © 2019 Worrall, Bravo-Cazar, Nilon, Fletcher, Robinson, Carr and Mitter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental set up for aphid-transmission of BCMV on full seedlings of N. benthamiana. N. benthamiana seedlings newly at 4–6 leaf stage were either sprayed with 1:2 BCMVCP-BioClay or left untreated 5 days prior to aphid inoculation. Six plants in the middle were infected with BCMV as source plants. Thirty starved aphids were applied per BCMV source plant 5 days post-spraying. Plant tray was maintained in an insect cage in growth cabinet conditions for 20 days.