FIG 4.

Children with recent clinical malaria episodes (n = 6) exhibited increased serological responses to three RIFINs (A and C) and six STEVORs (B and D) during the transmission season as measured using peptide microarrays. (A and B) Day 90 pediatric sera (maroon) recognized significantly more (A) RIFIN and (B) STEVOR peptides in total than the same day 0 pediatric sera from before the malaria season (blue). The x axis depicts the peptide number corresponding to the sequence from the N terminus to the C terminus for each respective antigen. ***, significant difference in serorecognized peptide counts between day 0 and day 90 (P < 0.05; McNemar’s test). (C and D) Day 90 sera (maroon) had increased seroreactivity to subsets of (C) RIFIN and (D) STEVOR peptides compared with matched sera before the malaria transmission season (blue) (P values in gray; Wilcoxon signed-rank test).