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. 2019 Mar 12;20(5):1241. doi: 10.3390/ijms20051241

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of proteome and phosphoproteome results by Western blot analysis. Primary human monocytes were incubated ± 400 U/mL TNF for 48 h. In whole cell extracts, protein levels of p52, v-rel reticuloendotheliosis viral oncogene homolog (REL) B, indolamin-2,3-dioxygenase (IDO), and kynureninase (KYNU) (A), as well as (p-)cluster of differentiation (CD) 44, (p-)vimentin (VIM) (indicated by an arrow), and (p-)myristoylated alanine-rich C-kinase substrate (MARCKS) (B), which were identified by LC-MS/MS as increasingly expressed or phosphorylated following 48 h TNF treatment, were determined using Western blot analyses (n = 3; representative experiments). Loading control: Actin.