Table 2.
Phosphoproteome: Top 25 peptides with significantly increased phosphorylation in TNF long term-incubated monocytes. Using LC-MS/MS analysis, the phosphoproteome in TNF long term-incubated and naive monocytes was analyzed. Subsequently, the ratio between phosphorylated peptides in TNF long term-incubated and control cells was calculated (phosphopeptide TNF/phosphopeptide control). The affected phosphorylation sites and gene functions are also given.
| Protein Name | Phosphopeptide Ratio | Phosphorylation Site | Function |
|---|---|---|---|
| EIF3G | 45.9 | T41 | gene regulation |
| ITGA5 | 42.4 | S128 | cell structure |
| MARCKS | 39.7 | S101 | cell structure |
| RBM14 | 35.2 | S620, S623 | gene regulation |
| VIM | 29.2 | S56 | cell structure |
| MTDH | 28.9 | S298 | cell structure |
| SRRM2 | 24.7 | S2067, T2069, S2071 | gene regulation |
| MARCKS | 22.7 | S77 | cell structure |
| ADAM17 | 21.3 | S791 | proteolysis |
| LAD1 | 20.3 | S38 | cell structure |
| OGFR | 19.6 | S577, S637 | gene regulation |
| SRRM2 | 18.6 | S2067, T2069, S2071 | gene regulation |
| FOSL2 | 18.2 | S308 | gene regulation |
| CD44 | 18.1 | S697 | cell structure |
| CD44 | 18.1 | S706 | cell structure |
| RELB | 16.7 | S573 | gene regulation |
| NUMA1 | 16.7 | S1757 | cell structure |
| RFTN1 | 16.6 | S199 | cell structure/signalling |
| CLIP1 | 16.3 | S195, S200 | cell structure |
| PRRC2C | 16.1 | S1544 | unknown |
| TRAF1 | 16.0 | S66 | signalling |
| PFKFB3 | 15.4 | S461 | metabolism |
| FKBP15 | 15.4 | S1114 | cell structure |
| LARP4B | 15.4 | S601 | gene regulation |
| PPFIA1 | 15.0 | S763 | cell structure |