Figure 3.

Analysis of HO-1 and GR subcellular localization. Immunofluorescence analysis of HO-1 and GR expression and localization in PC3 cells treated with Hemin (80 μM for 24 h), Dex (10 nM for 6 h post Hemin/PBS 24-h treatment), and the combination of both drugs or PBS as control. Cells were fixed, stained with anti-HO1, and anti-GR primary antibodies and secondary antibodies conjugated with Alexa Fluor 488 (red, HO-1) and 555 (green, GR) antibodies. Cells were imaged by confocal microscopy using the same parameters for all the treatments. A representative image for each condition is shown. Final magnification: ×60.