Figure 3.

Cellular and nuclear morphology in control cells and after PDT (photodynamic therapy). Cells were observed by phase contrast microscopy (PHC). (a) Control HeLa cells presented an epithelial aspect; after 24 h treatment a high number of rounded mitotic cells could be seen in the cultures; after 48 h treatment, cells with multinucleated morphology appeared in the cultures (asterisk) and apoptotic morphologies. Scale bar: 100 µm; inserts 10 µm. (b) PHC and nuclei morphology observed by fluorescence microscopy after Höechst-33258 staining, after 24 h PDT mainly metaphases, normal and abnormal with chromosome fragmentation and after 48 h PDT apoptotic morphology. (c) Cell cycle distribution outlines in each cell cycle phase 0, 24 and 48 h after PDT. Scale bar: 20 µm.