Table 1.
Comparison between T1 and T2 methylation levels of CPT1A and SREBF1 variably methylated sites (n = 52).
| Gene | Genomic Context | EpiTYPER CpG Unit | Mean Methylation (%) (SD) | p-Value * | |
|---|---|---|---|---|---|
| T1 | T2 | ||||
| CPT1A | Intron 1 | CpG_2 a | 1.9 (0.7) | 1.9 (0.6) | 8.2 × 10−1 |
| CpG_3 | 8.9 (1.3) | 9.1 (1.0) | 2.4 × 10−1 | ||
| CpG_4 | 4.5 (1.1) | 4.7 (0.9) | 7.3 × 10−2 | ||
| CpG_6 | 11.9 (3.8) | 12.5 (4.0) | 2.8 × 10−1 | ||
| CpG_7 | 4.8 (1.3) | 4.9 (1.1) | 3.6 × 10−1 | ||
| CpG_8 b | 6.5 (1.5) | 6.8 (1.3) | 1.4 × 10−1 | ||
| SREBF1 | Intron 1 | CpG_1 | 46.6 (2.8) | 47.2 (2.9) | 5.0 × 10−3 |
| CpG_3 | 36.9 (4.0) | 36.7 (4.2) | 7.5 × 10−1 | ||
| CpG_4 c | 71.3 (2.5) | 72.7 (2.3) | 1.6 × 10−7 | ||
| CPT1A | Exon 15 | CpG_2.3 d | 26.0 (13.3) | 29.1 (13.0) | 2.9 × 10−5 |
| CpG_5.6 | 48.5 (10.5) | 52.0 (10.1) | 2.3 × 10−6 | ||
| CpG_8.9 | 73.5 (5.7) | 75.2 (5.7) | 3.0 × 10−4 | ||
| CpG_10 | 38.7 (10.3) | 41.7 (9.9) | 3.3 × 10−5 | ||
| CpG_12 | 19.5 (5.8) | 21.9 (5.6) | 6.4 × 10−4 | ||
Values given are mean (SD) of the methylation in percentage. a—cg00574958, b—cg17058475, c—is a proxy for cg11024682, d—cg14249520. * p value for the difference between T1 and T2 methylation levels analyzed using a paired t-test. The correlation matrix for the methylation of all the CpGs within CPT1A intron 1, SREBF1 intron 1, and CPT1A exon 15 is shown in Table S3.