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. 2019 Mar 7;20(5):1163. doi: 10.3390/ijms20051163

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of SEPT1/6/7/11 on SPAG4 localization around the NE in the NT2/D1 male germ cell line (A) Western blot analysis of NT2/D1 cells transfected with the pEGFP-vector, pEGFP-SEPT1, pEGFP-SEPT7, pEGFP-SEPT11, or pEGFP-SEPT6 with pFLAG-SPAG4 using the anti-GFP antibody (anti-GFP Ab) and anti-FLAG antibody (anti-FLAG Ab). (B) Fold changes in disrupted SPAG4 localization in transfected cells with the pEGFP-vector, pEGFP-SEPT12, pEGFP-SEPT1, pEGFP-SEPT6, pEGFP-SEPT7, or pEGFP-SEPT11 with pFLAG-SPAG4. The height of the boxes represents the mean of values obtained from three independent experiments. At least 100 transfected cells were counted in each experiment (*** p < 0.001, Student t test).