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Effects of SEPT1/6/7/11 overexpression on the morphology of the NE in the NT2/D1 male germ cell line. Immunofluorescence staining with the anti-LAMIN antibody in the NT2D1 cells cotransfected with the pEGFP-empty (A), SEPT1 (B), SEPT6 (C), SEPT7 (D), or SEPT11 (E) vectors and pFLAG-SPAG4 vector. (a) Anti-EGFP antibody (green), (b) anti-FLAG antibody (pink), and (c) anti-LAMIN antibody (red); (d) image obtained after merging the images in (a), (b), and (c). Scale bar: 10 µm.
