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. 2019 Mar 6;20(5):1144. doi: 10.3390/ijms20051144

Figure 4.

Figure 4

FK506+minocycline treatment efficiently reduced astroglyosis in prion infected hamsters. (A) Representative immunohistochemistry pictures of cerebellum, medulla, midbrain, and cortex stained with GFAP antibody for activated astrocytes (scale bar-50 μm). (B) Representative immunohistochemistry pictures of cerebellum, medulla, midbrain, and cortex stained with IBA1 antibody for activated microglia (scale bar-50 μm). (C) Representative photos of Hematoxylin and Eosin (H&E) stain of cerebellum and medulla for vacuolation profile in prion infected and non infected animals. The bottom three slides show the dentate gyrus neurons in prion infected and non infected animals (scale bar-50 μm). (D) The graphical presentation of activated astrocytes in cerebellum, medulla, midbrain and cortex of 5 animals each per group, the data was analyzed by using 2 way ANOVA test followed by bonferroni post hoc test. (P ≤ 0.05 = *, P ≤ 0.01 = **, P < 0.001 = ***, P > 0.05 = ns). (E) The graphical presentation of activated microglia in cerebellum, medulla, midbrain and cortex of 5 animals per group. The data was analyzed by using 2 way ANOVA test followed bonferroni post hoc test. (P ≤ 0.05 = *, P ≤ 0.01 = **, P < 0.001 = ***, P > 0.05 = ns). (F) The graphical representation of vacuolation profile in prion infected and non infected groups. Number of animals per group was 5. The data was analyzed by using 2 way ANOVA test followed by bonferroni post hoc test. (P ≤ 0.05 = *, P < 0.001 = *** and P > 0.05 = ns). (G) Representative blots of two animals from prion infected and non infected groups for GFAP and IBA1 expression. (H) Graphical presentation of GFAP expression in prion infected and non infected animals. Values were normalized using GAPDH as loading control. Number of animals used for statistical analysis was 5 per group. The data was analyzed by using one way ANOVA test followed by post hoc test tukey’s multiple comparison. (P ≤ 0.05 = * and P < 0.0001 = ***). (I) Graphical representation of IBA1 expression in prion infected and non infected animals. Values were normalized using GAPDH as loading control. Number of animals used for statistical analysis was 5 per group. The data was analyzed by using one way ANOVA test followed by post hoc test tukey’s multiple comparison. (P < 0.0001 = *** and P > 0.05 = ns).