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. 2019 Mar 6;20(5):1144. doi: 10.3390/ijms20051144

Figure 9.

Figure 9

FK506+minocycline treatment increases cognition and survival via CREB and BAD phosphorylation. (A) Representative western blot panel of two animals each per group for total BAD and phosphorylated BAD levels. (B) Graphical presentation of expression level of phosphorylated BAD in different groups based on 5 animals data per group. Values were normalized using total BAD as loading control. The data was analyzed by using a one way ANOVA test followed by post hoc test tukey’s multiple comparison. (P ≤ 0.05 = * and P < 0.0001 = ***). (C) Representative western blot panel of two animals from each group for total CREB and phosphorylated CREB levels. (D) Graph showing protein expression level of phosphorylated CREB in different groups based on 5 animals data per group. Values were normalized using total CREB as loading control. The data was analyzed using a one way ANOVA test followed by post hoc test tukey’s multiple comparison. (P ≤ 0.05 = * and P < 0.0001 = ***). (E) Representative pictures of immunohistochemical analysis for the expression of LMN A/C protein in different groups (scale bar-50 μm). (F) Data showing the protein expression levels of LMN A/C for apoptosis analysis in different experimental groups based on 5 animals each per group. The data was analyzed by using a one way ANOVA test followed by post hoc test tukey’s multiple comparison. (P ≤ 0.05 = * and P < 0.0001 = ***).