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. 2019 Mar 4;24(5):896. doi: 10.3390/molecules24050896

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Alterations in the concentrations of CCK and gastrin in the colon of Lop + SpiA treated group. (A) The CCK concentration was measured in the colon homogenate by an enzyme-linked immunosorbent assay. The minimum detectable concentration of this kit is 0.1–1000 pg/mL CCK. (B) The gastrin concentration was measured in the colon homogenate by an enzyme-linked immunosorbent assay. The minimum detectable concentration of this kit is 0.312–20 pg/mL. Five-to-six mice per group were used in preparation of total lysate and hormone level was assayed in duplicate in each test. Data are reported as the mean ± SD. *, p < 0.05 compared to the untreated group. ^#, p < 0.05 compared to the Lop + Vehicle treated group. No, Untreated group; Lop, Loperamide; SpiA, Spicatoside A, CCK; Cholecystokinin.