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. 2019 Mar 6;20(5):1147. doi: 10.3390/ijms20051147

Figure 3.

Figure 3

Effect of FFE on cell cycle arrest and apoptosis in MDA-MB-231 cells. MDA-MB-231 cells treated with FFE for 24 h, 48 h, and 72 h. (A) Cell lysates prepared and subjected to Western blotting for cell cycle-related proteins (p21, CDK2, cyclin E, cyclin A, SKP2 and β actin). (B) Fold change of Western blot. Data represent mean ± SD, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with control. (C) Levels of apoptosis-related proteins (C-Cas9, C-Cas3, Bcl-2, PARP, C-PARP and β-actin) determined by Western blot analysis. Fold change of Western blot. (D) Data represent mean ± SD, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with control.