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. 2019 Mar 3;20(5):1091. doi: 10.3390/ijms20051091

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Extracellular protein profile and immunoblot analysis of Nops. Extracellular proteins produced by S. fredii USDA191 (lanes 1 and 2), RCB26 (lanes 3 and 4), B. diazoefficens USDA110 (lanes 5 and 6), and Δ136 (lanes 7 and 8) were resolved by 15% SDS-PAGE and silver stained (A,C) or transferred to nitrocellulose membrane for immunoblot analysis (B,D). Nops were detected with a cocktail of antibodies raised against individual Nop proteins. Odd and even number lanes contain proteins isolated from cells grown in the absence or presence of 1 µM apigenin or genistein, respectively. The size of the molecular weight markers and the identity of Nops are shown at the sides of the figure.