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. 2019 Mar 9;20(5):1206. doi: 10.3390/ijms20051206

Figure 7.

Figure 7

Activation of the WNT/β-catenin pathway by MARK4 promotes lipogenesis in pig primary trophoblast cells challenged with 400 μM NEFA. (A–D) Representative immunoblots and densitometric quantification for MARK4, DKK1 and β-catenin after transfection with Myc-MARK4, sh-MARK4 for 48 h in primary trophoblast cells isolated from pig placentas. Cells were then incubated with 400 μM NEFA or 10 μM JW74 for 24 h (n = 3). (E–H) Relative mRNA expression of lipid metabolism-related genes (E and F), fatty acid (FA) transporters (G) and regulators of lipid metabolism (H) after transfection with Myc-MARK4, sh-MARK4 for 48 h in primary trophoblast cells. Cells were then treated with 400 μM NEFA or 10 μM JW74 for 24 h (n = 3). Values are expressed as mean ± SEM.* p < 0.05 compared with the control group. Myc-MARK4 group: over expression of MARK4 group, sh-MARK4 group: knock down of MARK4 group, Control: empty vector (EV) group.