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. 2019 Mar 11;20(5):1216. doi: 10.3390/ijms20051216

Figure 2.

Figure 2

PSE inhibits adipogenesis in 3T3-L1 adipocytes. 3T3-L1 cells were seeded and induced to differentiation in the presence of DMSO (vehicle control), resveratrol (20–40 μM) or PSE (50–200 μg/mL) for 10 days: (A) TG accumulation in 96-well culture plates was visualized by ORO staining; (B) extracted ORO staining was quantified (OD 500 nm); (C) representative images from three separate experiments (magnified 4×); (D) adipogenic gene expression of PPARγ, aP2, C/EBP α, and Fas by qPCR; (E) adipogenic protein expressions of PPARγ, aP2, phosphor-specific, or total antibodies targeting AMPK and β-actin by Western blot analysis. All values are presented as the mean ±S.E.M. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 compared with the vehicle control (DMSO treated cells) by one-way ANOVA with Bonferroni’s comparison test. +; treatment, -; non-treatment.