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. 2019 Mar 7;24(5):946. doi: 10.3390/molecules24050946

Table 1.

Summary of binding affinity (KD, nM) for the interaction of representative compounds with biotin-labeled DNA sequences using biosensor-SPR method a.

graphic file with name molecules-24-00946-i001.jpg
Compounds X Y KD (nM)/Selectivity Ratio AAATTT KD (nM) AAAGTTT KD (nM)/Selectivity Ratio AAAGCTTT
DB2457 H Me 222/56 4 192/48
DB2708 H i-Pr 1020/255 4 223/56
DB2711 H graphic file with name molecules-24-00946-i002.jpg NB/-- 13 NB/--
DB2714 H graphic file with name molecules-24-00946-i003.jpg NB/-- 9 1330/148
DB2727 H graphic file with name molecules-24-00946-i004.jpg NB/-- 14 388/28
DB2740 H graphic file with name molecules-24-00946-i005.jpg NB/-- 3 107/36
DB2759 Cl i-Pr NB/-- 14 NB/--
DB2762 CF3 i-Pr NB/-- 94 NB/--

a All the results in this table were obtained in Tris-HCl buffer (50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 0.05% P20, pH 7.4) at a 100 μL/min flor rate. NB means no binding. The listed binding affinities are an average of two independent experiments carried out with two different sensor chips and the values are reproducibile within 10% experimental errors.