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. 2019 Mar 7;20(5):1164. doi: 10.3390/ijms20051164

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inactivation of Tie-2 enhances the cytotoxic effect of γ-T3. PC-3 cells were treated with different combinations of Tie-2 inhibitor and γ-T3. (A) The number of colonies formed were stained and counted after 14 days (A,B). Protein expression of cleaved PARP, total PARP, and actin (as a loading control) in PC-3 (C) and C42B (D) cells after treating with Tie-2 inhibitor/γ-T3 or both compounds was examined by Western blotting. Each experiment was repeated at least three times, and the results are presented as the mean ± SD with representative Western blots shown. (p values: * <0.05; *** < 0.0005).