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. 2019 Mar 7;20(5):1164. doi: 10.3390/ijms20051164

Figure 4.

Figure 4

Combination of Tie-2 inhibitor and γ-T3 lead to activation of AMPK. PC-3 (A) and C42B (B) cells were treated with the indicated compounds for 48 and 72 h, respectively. Western blotting was performed to determine the level of phospho-AMPKα (Thr172), total AMPK, LCBII, and actin in both PC-3 and C42B cells. Note that the combined effect of Tie-2 inhibitor and γ-T3 led to a clear induction of phospho-AMPKα and LCBII expression in both PC-3 and C42B cells. Each experiment was repeated at least three times with representative Western blots shown.