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. 2019 Mar 12;20(5):1242. doi: 10.3390/ijms20051242

Figure 4.

Figure 4

MDMA and NT colocalized with dopamine transporter (DT1). (A,B) Representative images (light microscopy, 40×) of MDMA/DT1 double immunostaining in the frontal cortex of rats receiving (A) saline (CTRL) and of rats receiving (B) MDMA (MDMA-exposed, sacrificed after 6 h). (C) Blown-up image of the dotted area highlighted in (B). (D,E) Representative images (light microscopy, 40×) of NT/DT1 double immunostaining in the frontal cortex of rats receiving (D) saline (CTRL) and in rats receiving (E) MDMA (MDMA-exposed, sacrificed after 6 h). (F) Blown-up image of the dotted area highlighted in (E). Scale bar for images in (A,B,D,E) = 50 μm. (G–I) Representative images (light microscopy, 40×) of DT1 (G-red staining)/NeuN (H-green staining) immunofluorescence and merged images with 4′, 6-diamidino-2-phenylindole (DAPI) (I-blue staining) in the frontal cortex. Scale bar for images in (G–I) = 100 μm. Arrows indicate a representative DT1/NeuN double-stained cell.