. 2019 Mar 2;20(5):1080. doi: 10.3390/ijms20051080

Table 2.

In vivo biodistribution and specificity 3 h p.i. as %ID/g. Female Balb/c nu/nu mice with BxPC-3 xenografts were injected with 2 µg of labeled affibody conjugate. For specificity test, protein dose was adjusted to 70 µg using non-labeled HER3-targeting affibody molecule. In vivo specificity of [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NOTA was previously demonstrated [15].

Organ	[⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NOTA	[⁶⁸Ga]Ga-Z₀₈₆₉₈-NOTA		[⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NODAGA		[⁶⁸Ga]Ga-Z₀₈₆₉₈-NODAGA
Organ	2 µg	2 µg	70 µg	2 µg	70 µg	2 µg	70 µg
Blood	0.22 ± 0.08	0.28 ± 0.04 ^e	0.27 ± 0.02	0.126 ± 0.004 ^e,g	0.11 ± 0.02	0.31 ± 0.06^g	0.24 ± 0.03
Sal. Glands	0.9 ± 0.3 ^b,d	2.2 ± 0.3 ^b,e	0.5 ± 0.1 ^a	1.0 ± 0.1 ^e,g	0.35 ± 0.09 ^a	1.8 ± 0.3 ^d,g	0.40 ± 0.04 ^a
Lungs	0.9 ± 0.3 ^b,d	1.7 ± 0.1 ^b,e	0.6 ± 0.1 ^a	0.94 ± 0.06 ^e,g	0.34 ± 0.06 ^a	1.6 ± 0.2 ^d,g	0.55 ± 0.09 ^a
Liver	2.2 ± 0.7 ^b,d	6.0 ± 0.6 ^b,e,f	3.0 ± 0.6 ^a	2.0 ± 0.2 ^e,g	0.51 ± 0.04 ^a	4.5 ± 0.6 ^d,f,g	1.5 ± 0.2 ^a
Spleen	0.3 ± 0.1	0.67 ± 0.06 ^e,f	0.72 ± 0.09	0.3 ± 0.01 ^e	0.21 ± 0.04	0.45 ± 0.6 ^f	0.39 ± 0.06
Stomach	0.9 ± 0.4 ^b,d	2.2 ± 0.3 ^b,e	0.5 ± 0.2 ^a	1.2 ± 0.2 ^e,g	0.32 ± 0.05 ^a	1.8 ± 0.4 ^d,g	0.48 ± 0.07 ^a
Small Intestine	2.0 ± 0.1 ^b,d	6 ± 2 ^b,e	0.8 ± 0.1 ^a	2.7 ± 0.4 ^e,g	0.4 ± 0.1 ^a	5.1 ± 0.8 ^d,g	0.7 ± 0.2 ^a
Kidney	138 ± 28 ^b,c,d	214 ± 22 ^b,e	278 ± 44 ^a	324 ± 11 ^c,e,g	275 ± 48 ^a	237 ± 24 ^d,g	299 ± 43 ^a
Tumor	1.9 ± 0.7 ^b,d	3.9 ± 0.6 ^b,e	1.1 ± 0.2 ^a	1.9 ± 0.2 ^e,g	0.7 ± 0.1 ^a	3.3 ± 0.5 ^d,g	0.9 ± 0.2 ^a
Muscle	0.12 ± 0.08	0.19 ± 0.04	0.23 ± 0.10	0.11 ± 0.04	0.13 ± 0.05	0.18 ± 0.04	0.16 ± 0.05
Bone	0.32 ± 0.09	0.42 ± 0.08	0.4 ± 0.3	0.4 ± 0.2	0.19 ± 0.04	0.42 ± 0.1	0.4 ± 0.2
GI (% ID)	4 ± 1 ^b,d	9.3 ± 0.4 ^b,e,f	1.7 ± 0.8 ^a	4 ± 1 ^e,g	0.9 ± 0.4 ^a	7 ± 1 ^d,f,g	1.3 ± 0.6 ^a
Body (% ID)	6 ± 2 ^b,d	13 ± 3 ^b,e,f	5.4 ± 0.7 ^a	7 ± 1 ^e	4.1 ± 1.0 ^a	9.2 ± 0.8 ^d,f	5.0 ± 0.8 ^a

Statistical analysis was performed using one-way ANOVA with multiple comparisons using Bonferroni correction. Differences were significant (p < 0.05) between ^a 2 µg and 70 µg, ^b [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NOTA and [⁶⁸Ga]Ga-Z₀₈₆₉₈-NOTA, ^c [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NOTA and [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NODAGA, ^d [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NOTA and [⁶⁸Ga]Ga-Z₀₈₆₉₈-NODAGA, ^e [⁶⁸Ga]Ga-Z₀₈₆₉₈-NOTA and [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NODAGA, ^f [⁶⁸Ga]Ga-Z₀₈₆₉₈-NOTA and [⁶⁸Ga]Ga-Z₀₈₆₉₈-NODAGA, ^g [⁶⁸Ga]Ga-(HE)₃-Z₀₈₆₉₈-NODAGA and [⁶⁸Ga]Ga-Z₀₈₆₉₈-NODAGA.