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. 2019 Mar 6;20(5):1133. doi: 10.3390/ijms20051133

Figure 1.

Figure 1

Experimental flow of oocyte retrieval and in vitro maturation. Oocytes not pre-treated with gonadotropins were retrieved during follicle isolation. Immediate in vitro maturation of ruptured and retrieved oocytes was conducted in six different conditions; (1) human chorionic gonadotropin (hCG) 60 IU/L + FSH 200 mIU/mL in MEM α, (2) hCG 60 IU/L + epidermal growth factor (EGF) 5 mg/mL in MEM α, (3) hCG 100 IU/L + EGF 5 mg/mL in MEM α, (4) hCG 60 IU/L + FSH 200 mIU/mL in Universal IVF media, (5) hCG 60 IU/L + EGF 5 mg/mL in Universal IVF media, and (6) hCG 100 IU/L + EGF 5 mg/mL in Universal IVF media. Additionally, the collection of sperm by ejaculation using a vibrator from male marmoset is shown (left), and the collection of female ovaries by surgical operation under anesthesia is represented (right).