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In vitro fertilization of marmoset oocytes using different conditions. (A) Fertilization and development into the four-cell-stage. In vitro fertilization (IVF) was achieved using viable sperm, and fertilization was confirmed by observation of 2-pronuclei (2PN). In vitro development into the further stage was confirmed after 48 h. (B) Fertilization rate using each condition of hormone combinations. The rates were calculated based on the appearance of 2PN after IVF.
