Figure 2.

(a) Thermal melting temperatures for tC-modified probes: 8tC (orange), 14tC (yellow), 17tC (green), 19tC (blue), and unmodified 5′-(C₄G)₃C₄A-3′ sequence (black) plotted against pH. All values and standard errors are presented numerically in Table 2; (b) Denaturation normalized profiles of the 8tC probe recorded at 300 nm at three different pH values: pH 5.75 (green dash line); pH 6.25 (red dash line); pH 6.75 (black dash line). The presented curves are obtained during heating.