Figure 4.

Members of the TBX2 subfamily are hypermethylated in NSCLC cell lines. Methylation levels of members of the TBX2 subfamily were examined in eight NSCLC cell lines and normal alveolar epithelial cells by quantitative methylation-specific PCR using the Epitect Methyl II PCR assay from QIAGEN according to the manufacturer’s instructions (see Section 4). Percentages of un-methylated (UM) and methylated (M) TBX2 subfamily alleles are depicted and represent an average of two experiments (* p < 0.05 comparing methylated versus unmethylated fractions for each cell line).