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. 2019 Mar 11;20(5):1230. doi: 10.3390/ijms20051230

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differential kinase phosphorylation was observed between MV4-11-P and MV4-11-R cells. (A) Human phospho-kinase array blots showed increased (shown in red) and decreased (shown in blue) phosphorylation of multiple kinases in MV4-11-R compared with MV4-11-P. Spots for each phospho-kinase antibody are in duplicate. (B) Average pixel densities of duplicate spots on the human phospho-kinase array blots were analyzed using ImageJ. (C) Cell lysates of MV4-11-P and MV4-11-R were analyzed for selected total/phosphorylated kinases. Representative Western blots of three independent experiments are shown.