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. 2019 Mar 5;24(5):910. doi: 10.3390/molecules24050910

Table 5.

Inhibition of lipid peroxidation IC50 of compounds DAC, DACH, DiMeOC, DAC-Cu, DACH4-Cu and DiMeOC-Cu.

Products Concentration
(μM)
TBARS
(nmol/mg prot.)
Inhibition
(%)
IC50
(μM)
Basal - 0.24 ± 0.09 - -
FeSO4 100 µM - 9.15 ± 0.32 - -
α-Tocopherol
(n = 4)
0.1
0.32
1
3.16
10
31.62
100
6.28 ± 0.18
6.04 ± 0.24
5.21 ± 0.33 *
3.67 ± 0.56 **
2.72 ± 0.33 **
1.84 ± 0.31 **
1.40 ± 0.36 **
4.62 ± 0.57
8.26 ± 1.31
21.13 ± 2.56 *
44.84 ± 6.74 **
59.00 ± 3.71 **
72.3 ± 3.87 **
79.09 ± 4.79 **
6.78 ± 2.16
DAC 0.1
0.32
1
3.16
10
9.68 ± 0.43
9.29 ± 0.67
7.78 ± 0.53 *
5.22 ± 0.35 **
1.43 ± 0.08 **
4.16 ± 1.97
8.32 ± 1.49
23.19 ± 0.92 *
48.46 ± 1.53 **
85.82 ± 0.60 **
3.21 ± 0.16
DAC-Cu 0.1
0.32
1
3.16
10
9.08 ± 0.66
8.04 ± 0.70
6.24 ± 0.71 **
3.08 ± 0.21 **
1.32 ± 0.48 **
10.34 ± 1.48
20.78 ± 2.44
38.70 ± 3.82 **
69.57 ± 1.56 **
86.58 ± 5.21 **
1.55 ± 0.15
DACH4 1.78
3.16
5.62
10
17.78
31.62
56.23
8.32 ± 0.31
7.85 ± 0.41 *
7.25 ± 0.36 **
6.32 ± 0.18 **
4.45 ± 0.22 **
0.36 ± 0.06 **
0.14 ± 0.01 **
9.05 ± 1.03
14.28 ± 1.59 *
20.86 ± 1.48 **
30.84 ± 0.57 **
51.41 ± 0.92 **
96.02 ± 0.57 **
98.39 ± 0.18 **
16.46 ± 0.30
DACH4-Cu 1.78
3.16
5.62
10
17.78
31.62
56.23
7.32 ± 0.30 **
6.67 ± 0.34 **
5.89 ± 0.33 **
3.28 ± 0.37 **
0.28 ± 0.05 **
0.20 ± 0.02 **
0.14 ± 0.007 **
19.93 ± 2.01 **
27.14 ± 1.30 **
35.74 ± 1.88 **
64.27 ± 2.74 **
96.90 ± 0.63 **
97.73 ± 0.29 **
98.39 ± 0.03 **
7.93 ± 0.41
DiMeOC 17.78
31.62
56.23
100
177.83
5.51± 0.13 **
3.40 ± 0.007 **
2.67 ± 0.06 **
2.27 ± 0.09 **
2.08 ± 0.10 **
38.66 ± 3.26 **
62.16 ± 1.18 **
70.33 ± 0.63 **
74.75 ± 0.92 **
76.91 ± 0.88 **
23.01 ± 1.37
DiMeOC-Cu 1
3.16
10
31.62
100
316.23
8.58 ± 0.28
7.42 ± 0.20 **
4.28 ± 0.06 **
3.10 ± 0.12 **
2.89 ± 0.16 **
2.8 0± 0.23 **
4.55 ± 4.35
17.51 ± 2.63 **
52.41 ± 1.20 **
65.56 ± 1.49 **
67.90 ± 1.17 **
68.97 ± 1.99 **
9.35 ± 0.34

Data presented as mean ± SEM of three replicates. * p ≤ 0.05 and ** p ≤ 0.05 compared to FeSO4.