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EsHKT-RNAi plants are sensitive to salt stress. Wild type and knock-down lines of EsHKT1;2 (EsHKT-RNAi) were grown on MS-medium for 2-weeks and then transferred to inert soil (porous soil, see Ali et al. 2012 for details) and grown for further 3-weeks. Plants were then treated with 300 mM NaCl for another 3-weeks period, twice a week (control represents untreated plants). EsHKT-RNAi lines were more sensitive to salt stress as compared with wild type Control.
