Figure 6.

Experimental design: while non-cryo neurons were directly seeded after isolation, dissociation and counting, cryo-neurons were cryopreserved an thawed after 3 months. The day the neurons were seeded represents day 0 of the experiment. Part of the cultures were fixed for comparative immunofluorescence and morphometric analysis at 6 DiV for non-cryo and cryo neurons and at an additional time-point (12 DiV) for cryo neurons. The remaining DRG neuronal cultures were infected with 2 different strains of CDV (CDV-5804 PeGFP, CDV R252) at 6 DiV and kept in culture until 12 DiV or 6 dpi, respectively, when they were processed for immunostaining and further analysis. Non-cryo = non-cryopreserved neurons, cryo = cryopreserved neurons, DiV = days in vitro, dpi = days post infection, CDV = canine distemper virus, eGFP = enhanced green fluorescent protein.