MCSF	Macrophage colony-stimulating factor
RANK-L	RANK-Ligand
TNF	a Tumor necrosis factor alpha
OPG	Osteoprotegerin
PBMCs	Peripheral mononuclear cells
a-MEM	a-Minimal Essential Medium
DMEM	Dulbecco’s Minimal Essential Medium
PBS	Phosphate-buffered saline
RT	Room temperature
TRAP	Tartrate-resistant acid phosphatase