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pH-dependent hemolytic activities of all carriers at N/P ratio of 10. Rat blood cells were diluted 1:50 in PBS and incubated with each formulation at pH = 7.4, 6.5, and 5.4 for 2 h at 37 °C. Triton X-100 (1% v/v) was implemented as a positive control. Blank was rat blood cells incubated with PBS solutions. Data were subtracted by the average of blank readouts and normalized to positive control.
