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. 2019 Feb 27;20(5):1027. doi: 10.3390/ijms20051027

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sphere culture method enriches aldehyde dehydrogenase^hiCD44^hi HeLa cervical cancer stem-like sphere culture model (ALDH^hiCD44^hi hCSCs) population from parental HeLa cells. (a) Representative fluorescence-activated cell sorting (FACS) dot plots showing enrichment of ALDH^hi cancer stem-like cells from day 0 to day 10 post sphere formation in low anchorage dishes. The ALDH^hi gated cells were mostly CD44 positive (b). Bar graphs representing aldehyde dehydrogenase enrichment in HeLa cultures showing cancer stem cells enrichment level was significantly higher by end of the tenth day. All data represent means ± SEM, *** p ≤ 0.001. DEAB: diethylaminobenzaldehyde.