Figure 3.

Phenethyl isothiocyanate (PEITC) induces reactive oxygen species (ROS) in HeLa cancer stem cells (hCSCs). (a) Representative fluorescence-activated cell sorting (FACS) histograms showing PEITC induces ROS in hCSCs in 3 hr, which can be replenished by exogenous glutathione (GSH). H₂O₂ was used as a positive control (b). Bar diagram showing the ROS induction by PEITC in hCSCs. All data represent means ± SEM. One-way ANOVA, followed by a Dunnett’s post hoc test, was used to compare multiple means. The pairs with significant mean differences are shown, *** p ≤ 0.001. DCF-DA: dichlorofluorescin diacetate.